The unique EV-associated proteins detected in this studyinclude several conventional EV markers, proteins associated withEV biogenesis, EV surface, EV sorting/transport, and many cancer-relatedproteins (such as RAP1B, FLOT1/2, Annexins, RAB proteins, SLC2A1,SLC7A5, PSMD1, CKAP4, LEF1, PAX5, and TCL1A), which were not capturedin the plasma proteome or when performing conventional LG LC–MS/MSanalysis. This evidence concerns the gene LEF1 and cancer.