FOXO3 and immunodeficiency disease: Meanwhile, theWestern blot results also showed a downward trend in the levels ofkey functional proteins, including IL-7, IL-7R, SCF, c-KIT, GABPA,FOXO3A, and LYL1, which is consistent with the observed mRNA trends.Given the reduction in IL-7 positive cells and the significant downregulationof IL-7 signaling-related genes and proteins, the proliferation, development,and T-cell lineage commitment are markedly impaired, ultimately resultingin immunodeficiency.