To determine which among AFP and the 92 metabolites associated with HCC diagnosis within 12 months of follow-up contributed the most to risk prediction, conditional inference random forest was again implemented, including age, gender, PNPLA3 rs738409 GG, and TM6SF2 rs58542926 CT/TT genotypes (Fig S6 and Fig. 3A). This evidence concerns the gene PNPLA3 and hepatocellular carcinoma.