Subsequently, we conducted a more in-depthanalysis of the three-gene methylation patternbetween cancer-adjacent tissues and different molecularsubtypes and pathological grades of breast cancer.The methylation level of the RANBP3 gene inHR+/HER2-, HR+/HER2+, and HR-/HER2+ typessignificantly differed between cancer-adjacent tissues and cancer tissues (P=0.000, P=0.000, P=0.013,Tukey HSD). This evidence concerns the gene HR and breast carcinoma.