In summary, findings of this study demonstrated that the combination of miRNAs including miR-151a-3p or miR-21-5p or miR-126-5p along leptin and miR-151a-3p with glucose can be used as non-invasive biomarker for the comprehensive assessment of steatosis, liver stiffness and hepatic fat content, which are critical factors in determining the presence of MASLD. Here, LEP is linked to metabolic dysfunction-associated steatotic liver disease.