As expected, MCF10DCIS.com cells that overexpress SEMA3F exhibit a noticeable increase in both NRP1 and NRP2 expression in 3D cell cultures, which is more evident for NRP1 (Fig. 2f), and with a localization compatible with myoepithelial cell distribution in 3D BC cell cultures. The gene discussed is NRP2; the disease is breast cancer.