After 72 h of selection at 1 μg/ml doxycycline to achieve potent SEMA3F expression activation in MCF10DCIS_SEMA3F cells (Supplementary Fig. 3a), the effects of overexpression on DCIS progression to IDC were tested by in vitro and in vivo experiments (Figs. 2 and 3). This evidence concerns the gene SEMA3F and ductal breast carcinoma in situ.