Consistent with RNA velocity analysis (Fig. 1F), pseudotime analysis using Monocle3 predicts a trajectory from the six2a + OM cluster to mmp9+, spp1+ pre-osteoblasts; spp1+, ifitm5+ early osteoblasts; and bglap1+ late osteoblasts (Fig. 3A-C). The gene discussed is IFITM5; the disease is ocular melanoma.