CDK5RAP2 and infection: Silencing endogenous Cdk5rap2 expression in the primary culture of mNPCs in vitro by lentivirus (pPurGreen) infection in 48-h culture resulted in a significant decrease in the intensity of Cdk5rap2 at the centrosomes in metaphase mNPCs (shCdk5rap2-1 = 115.6 A.U.; shCdk5rap2-2 = 107.5 A.U., respectively) as compared to control (Fig 7B and 7C; metaphase: 153.0 A.U.).