To our surprise, MBP-fusions to the BHF of Jonah-1, CuRO-1 of laccase-1, and the entire Luke-2 and Leo-A minus their signal peptides all bound in a similar pattern to both layers of the cyst wall, suggesting that cellulose and/or chitin, to which each recombinant wall protein binds, are present in both layers (22, 24, 27, 41, 42). Here, MBP is linked to cyst.