To further validate that the lipid scrambling can preserve the plasma membrane integrity upon Listeria infection, mouse TMEM16A, the authentic TMEM16 family member with solely the chloride channel function, was fused with the previously identified scramblase domain (SCRD) from TMEM16F, forming SCRD chimeras.[49] SCRD chimeras largely restored the lipid scrambling function triggered by ionomycin in TMEM16F KO RMA cells (Figure S2F, Supporting Information). Here, ANO6 is linked to listeriosis.