To delve deeper into the specifics of how TMEM16F modulates plasma membrane lipids in response to intracellular bacterial infection‐induced PM damage, we initiated our investigation using Laurdan dye, a fluorescent probe that reflects plasma membrane lipid order.[50, 51] Intriguingly, we observed a pronounced decrease of Laurdan signal in living scrambled cells (Annexin V+ PI−) during Listeria infection, a phenomenon not witnessed in TMEM16F‐deficient cells (Figure3A). This evidence concerns the gene ANXA5 and listeriosis.