As a facultative intracellular bacterium, L. monocytogenes primarily infects Kupffer cells (KCs), during the early stage of infection.[12] In Listeria infection, KCs undergo cell death and subsequently re‐populate from monocytes recruited into the liver.[12] Surprisingly, we found a more pronounced depletion of KCs (F4/80+Ly6CloCD11blo) in the absence of TMEM16F compared to their control littermates at 3 dpi, at both percentage and absolute number (Figure 6G; Figure S8A,B, Supporting Information). The gene discussed is ANO6; the disease is listeriosis.