Several ways of cell death for host cells have been reported during Lm infection, including cell‐intrinsic programmed cell death necroptosis, pyroptosis, apoptosis, and cell‐extrinsic death.[9, 12, 13, 33] However, in our in vitro experimental conditions, we observed that cell death in the absence of TMEM16F primarily resulted from the membrane damage caused by LLO, and a short period of time infection with Listeria has not initiated significant death in WT cells yet. Here, ANO6 is linked to infection.