Of note, low concentration of DAPI used in this study could only stain cells with the permeabilized PM, same as PI (Figure S1C, Supporting Information).[30] When co‐cultured with an increasing multiplicity of infection (MOI) of Lm, wild‐type (WT) thymocytes displayed more PS exposure, exhibiting substantial resistance to Listeria‐induced cell death, especially at low MOI of infection, whereas loss of TMEM16F led to significantly greater cell death (Figure S1D, Supporting Information). The gene discussed is ANO6; the disease is infection.