The higher number of seeding locations (≥ 3) showed the downregulation of PTGDS in the tumor (log2FC =  − 0.42, adjusted p-value < 0.001) and stromal regions (log2FC =  − 0.31, adjusted p-value < 0.001) and ALPL in the tumor region (log2FC =  − 0.48, adjusted p-value < 0.001, Table 3). The gene discussed is ALPL; the disease is neoplasm.