Next, to exclude potential off-target effects of BMX-IN-1 in NB spheroids, we surveyed the RNA-Seq data from both spheroids and adherent cell populations for the expression levels of other kinases that are potentially also targeted by BMX-IN-1, although with much lower potency (40), such as BTK, BLK, JAK3, EGFR, ITK, and TEC. The gene discussed is JAK3; the disease is neuroblastoma.