To identify the major transcriptional differences in immune cell populations with and without CpG and RT, we performed single-cell RNA-Seq (scRNA-Seq) on CD45+ tumor-infiltrating immune cells from an independent cohort of sarcomas sorted by FACS and harvested 6 days after 0 or 20 Gy RT and 3 days after Control or CpG (Figure 3A). This evidence concerns the gene PTPRC and sarcoma.