Although the expression of specific genes after combination CpG and RT treatment does not always differ significantly from tumors treated with CpG alone or RT alone, it is the combination of changes after RT, including increased TLR9 expression with the TLR9 agonist CpG, that is necessary for maximal CD8 T cell infiltration, which is required for maximal tumor response. This evidence concerns the gene TLR9 and neoplasm.