As in Fig. 8B, IF staining further supported these findings, which showed that occludin-1 and claudin-1 proteins were absent in colitis tissues but were localized in normal intestinal epithelial cell membranes after XYKJP treatment, suggesting that XYKJP can promote the repair of intestinal barrier function by increasing the expression of TJ proteins. The gene discussed is CLDN1; the disease is colitis.