IFNG and tuberculosis: The incidence of TB in buffalo herds increased from 1.19% in 2016, 2.81% in 2017, and 2.39% in 2018, to a peak of 6.27% in 2019 after 3 years of TB control with the IFN-γ test, as introduced by new regional legislation (DD 226/2016) (Figures 5, 6).