IFNG and tuberculosis: In 2015, additional studies (IZS ME 06/15 RC) (13) were conducted under the project “Study on the specificity of the intradermal test in the buffalo species (Bubalus bubalis): interfering microbial agents and involved immune mechanisms.” The project aimed to assess the presence of nonspecific reactivity in negative animals from Officially Tuberculosis-Free (OTF) herds and compare the specificity of SIT and CIT in the buffalo species, and thereby enrich the data that had already been acquired from previous studies on the use of the IFN-γ test (14).