In silico analysis predicted distinctive roles of these transcripts in the cell that could explain their opposite levels in CRC: SMAD4-209 is coding for full-length SMAD4 protein - a major regulator of homeostasis and cell cycle in healthy tissue, while SMAD4-213 is coding for a truncated protein that could affect highly regulated TGF-β signaling and push the cell into homeostatic dysregulation, and has a predicted secondary structure with potential for sponging miRNAs with tumor suppressive roles in CRC. Here, SMAD4 is linked to colorectal carcinoma.