COA6 mRNA and protein amounts were assessed in MDA-MB-231, HCC1937, SK-BR-3 and MCF-7 (breast cancer cells) comparatively to the noncancerous breast ductal epithelial MCF10A cell line using qRT-PCR (Figure 4A) and immunoblot (Figure 4B). The gene discussed is COA6; the disease is breast carcinoma.