To examine the transcriptional changes in endothelial cells (EC) within well-developed atherosclerotic plaque, we employed a model of low flow induced by Partial Carotid Arterial Ligation (PCAL) coupled with hyperlipidemia mediated by adenoviral delivery of mutated proprotein convertase subtilisin/kexin type 9 (PCSK9) (AAV-mPCSK9) and high fat diet27. This evidence concerns the gene PCSK9 and hyperlipidemia.