In addition to the therapies listed above, researchers are working on the treatment of glioblastoma with cytotoxic precursor drugs, angiogenesis inhibitors, PI3K/Akt/mTOR pathway inhibitors, matrix MMP inhibitors, and other drugs.699–702 At present, the toughest challenges in the diagnosis and treatment of glioblastoma mainly lie in the following aspects: the blood-brain barrier, perivascular/perineural microinvasion, hypoxia microenvironment, drug resistance, intratumoral molecular heterogeneity, etc.632,703,704 We will present the below contents from the facing difficulties. This evidence concerns the gene AKT1 and glioblastoma.