However, the involvement of Acin1 in HCC has not been reported yet, so this study analyzed the expression of Acin1 mRNA in HCC by whole transcriptome sequencing, bioinformatics analysis, and reverse transcription-quantitative polymerase chain reaction (RT-qPCR), further screened the differentially expressed Acin1-related genes in tumor tissues, constructed protein–protein interaction (PPI) and competitive endogenous RNA (ceRNA) regulatory networks, etc., and further analyzed their functions and interactions through online databases to predict the targeting and regulation of Acin1. The gene discussed is ACIN1; the disease is neoplasm.