We will further screen the differentially expressed Acin1-related genes in tumor tissues, construct PPI and competing endogenous RNA (ceRNA) regulatory networks, analyze their functions and interactions, and further predict the miRNAs targeting Acin1 through online databases to elucidate the possible molecular mechanisms of Acin1 gene aberrant expression in HCC. Here, ACIN1 is linked to hepatocellular carcinoma.