The 5XFAD mouse model was created in 2006 and expressed excess amounts of human PSEN1 protein with two Familial AD (FAD) mutations (M146L and L286V), as well as human APP with three FAD mutations: the Swedish (K670N, M671L), London (V7171), and Florida (I716V) mutations [169]. This evidence concerns the gene PSEN1 and Alzheimer disease.