Then, they evaluated the response under a 10-day incubation with a combination of different toxic stress factors (high glucose concentration (25 μM), insulin (6.9 nM), and non-esterified fatty acids (NEFAs): oleic acid (65 μM) + palmitic acid (45 μM)) comparing the outcome with clinical data from NAFLD and NASH patient biopsies. The gene discussed is INS; the disease is metabolic dysfunction-associated steatotic liver disease.