GPT and steatosis: In mice suffering from carbon tetrachloride (CCl4)-induced hepatitis, disease severity—comprising hepatic injury, steatosis, and elevated serum levels of alanine (ALT) and aspartate aminotransferases (AST)—could be significantly attenuated, when the animals were pre-treated with methylene blue, a substance that concomitantly reduced the net activity of GSK3β in the liver [113].