TNF and adrenal cortex carcinoma: GSEA enrichment analysis of PYCRs showed that in GBM, PRAD, and SKCM, PYCR1 negatively regulates TNF-α signaling and IL6-JAK-STAT3 signaling, while in ACC, KIRC, KIRP, and THCA, PYCR1 positively regulates G2M checkpoint, EMT, and E2F targets (Figure 1C).