MKI67 and steatosis: We used hematoxylin and eosin (H&E) staining to assess steatosis and inflammation, immunohistochemical (IHC) staining of Ki-67 to assess hepatocyte proliferation [62], and IHC staining of p62 as a marker of liver damage, chronic steatohepatitis, and risk for hepatocellular carcinoma (HCC) onset and progression [34,35,63,64].