CASP3 and cancer: Apoptosis and necrosis of cultured cancer HeLa cells were detected by immunohistochemistry for caspase-3 at bright microscopy using biotinylated antibodies as well as by fluorescent DNA staining (4′,6-diamidino-2-phenylindole also known as DAPI, Hoechst, propidium iodine) to evaluate the efficacy of treatment with (poly(3-hydroxybutyrate-co-3-hydroxyhexanoate)-based NPs containing the antineoplastic agent etoposide [83].