The above results indicated that AGAP2-AS1 promoted the proliferation, migration and invasion of glioma cells mainly through regulating miR-15a/b-5p/HDGF/Wnt/β-catenin, EZH2/LSD1/TFPI2, miR-9-5p-MMP-2/MMP-9 and NF-κB/Erk1/2 pathways. Here, AGAP2 is linked to central nervous system cancer.