We evaluated the impact of free drugs (Si, 5-FU), free polymeric matrices (HA-FA, CMCH-DA), empty MSNs, and all typologies of nanoparticles (5-FU/MSN, HA-FA/5-FU MSN, CMCH-DA/5-FU MSN, Si/MSN, HA-FA/Si MSN, and CMCH-DA/Si MSN) on breast cancer cell viability using ER-α positive MCF-7 on glioblastoma cell line T98G. The gene discussed is ESR1; the disease is breast cancer.