Interference with many signaling pathways, such as NOD-like receptor signaling pathway, Herpes simplex infection, Hepatitis C, PI3K-Akt signaling pathway, and many factors, such as Atp13A4 and Gbgt1, affect the proliferation and apoptosis of 3T3-L1 preadipocytes. This evidence concerns the gene AKT1 and herpes simplex infectious disease.