We further discuss the roles of glycosylation in tumorigenesis, progression and metastasis, together with the role of several well-known cancer-associated cell signaling pathways such as epidermal growth factor receptor (EGFR), phosphatidylinositol 3-kinase/protein kinase B (PI3K/Akt) and p53 pathways affected by glycosylation. This evidence concerns the gene EGFR and cancer.