The differential protein markers that were identified as promising protein targets by the proteomics analysis: CD63, CD166, S100A13, and TNF-β, as well as the target proteins of the differentially expressed miRNA-1285: c-Jun, p53, CDH-1, Smad4, TGM2, and YAP, were analyzed/validated via Western blotting within the two disease groups PDAC and PDAC + DM. This evidence concerns the gene SMAD4 and diabetes mellitus.