To investigate the cytotoxicity of the AQP inhibitors Auphen (pan-AQP inhibitor) and Z433927330 (AQP7 inhibitor) (Figure 1A), a panel of mouse and human breast cancer cell lines were cultured and treated with different concentrations of Auphen or Z433927330, and the cytotoxic effects were assessed using CellTiter-Glo assay (Figure 1B,C). This evidence concerns the gene AQP7 and breast cancer.