To assess the protein levels of HMGA2 and AR in PCa cells, we conducted Western blot analysis across various PCa cell lines, including parental LNCaP originally derived from lymph node metastasis, C4-2B, a bone metastatic subline of LNCaP, and enzalutamide-resistant C4-2B cells (C4-2B MDVR). Here, HMGA2 is linked to posterior cortical atrophy.