As the above results suggested the importance of RGS1 in the function of macrophages against MTB, we transfected RGS1 small interfering RNA (siRNA) (named RGS1-KD) into cultured THP-1-derived macrophages, followed by H37Rv infection at a multiplicity of infection (MOI) of 10 for 24 h. This evidence concerns the gene RGS1 and infection.