AFF2 and HIV-1 infection: These three are ALF transcription elongation factor 2 (AFF2) mRNA interactor of Tat, 60S acidic ribosomal protein P0 (RPLP0) mRNA interactor of both Nucleocapsid and Rev, and H4 clustered histone 9 (H4C9) mRNA detected as interacting with Nucleocapsid, Gag, and Rev. Knockdown was confirmed with qPCR both before and 48 h post HIV-1 infection (Fig. 5B).