Second, for three HIV-1 proteins (Nucleocapsid, Matrix, Tat) demonstrating the strongest host RNA interactions, we created a second cell line of interest (SupT1 cells) and performed FLAG-based nRIPseq, both in the presence and absence of an HIV-1 infection (Assays C and D; Appendix Figs. S3,, S4). The gene discussed is TAT; the disease is HIV-1 infection.