As the Hippo pathway and its effector protein YAP, a transcriptional coactivator, have been identified as important signaling cascades in regulating gene function, we then investigated whether HFD-induced oxidative stress affects YAP and the Notch1 intracellular domain (NICD) in the progression of NASH. Here, NOTCH1 is linked to metabolic dysfunction-associated steatohepatitis.