Next, to confirm scRNA-seq findings and assess potential inter- and intra-tumor heterogeneity of anti-apoptotic gene expression, we visualized gene expression spatially using Visium analysis of sections from lung-specific EGFR-L858R-T790M (EGFRTL/CCSP-rtTA) transgenic mice to compare samples that were (1) untreated, (2) treated short-term to represent the DTP state, and (3) treated long-term to represent the recurrent state. This evidence concerns the gene EGFR and neoplasm.