PRAME and neoplasm: This hypothesis is supported by the fact that CAR-T cells containing other variants, such as 98I (mutation R63, R65, and R72), (KD = 0.74 nM, on-rate = 150 × 105/Ms, and off-rate = 1.14 × 10−2/s) and 98D (mutation R63, R70, and R72), (KD = 2.3 nM, on-rate = 78 × 105/Ms, and off-rate = 1.76 × 10−2/s) were poorly activated in T2A24 cells pulsed with the indicated PRAMEp301 peptides and PRAME/HLA-A∗24:02+ tumor cell lines, despite their high affinity (data not shown).