Considering our previous study, wherein we demonstrated MYD88-dependent induction of proinflammatory cytokines (IL-1β and TNF-α) from MФs after stimulation with CNTs [22], tracking diffusible soluble mediators in the treated macrophage culture supernatants in the ALI model could have expanded information on the induced inflammatory milieu. This evidence concerns the gene IL1B and acute respiratory distress syndrome.