A wide range of well-known tumor-mutated proteins, including four tumor neoantigens, Ddx27, Cad, Aldh18a1 and Glud1, were able to be adsorbed on the surface of antigen-capturing bacteria, eliciting antitumor responses by activating dendritic cells in the margins of the TME, which in turn migrated to the lymph nodes for T cell priming. Here, DDX27 is linked to neoplasm.