CD8A and neoplasm: Similarly, the intratumoral administration of the protozoan parasite Neospora caninum in mice bearing melanoma B16.F10 tumors led to augmented CD8+ T cell and CD68+ macrophage infiltration in the TME, accompanied with increased levels of Th1 cytokines in the tumor milieu, such as IFNγ, TNFα, IL-2, IL-10 and IL-12, therefore resulting in a reduction in tumor volume [94].