In this line, the intratumoral injection of the attenuated rhino/poliovirus chimera, PVSRIRO, in combination with intraperitoneal anti-PD-L1 administration, in mice bearing B16.F10 melanoma tumors led to TME enrichment not only in CD4+ T cells, but also in cytotoxic populations of NK and CD8+ T cells that are characterized by increased granzyme B and IFN-γ secretion rates. The gene discussed is IFNG; the disease is melanoma.