Modified species of higher molecular weight above the band corresponding to SQSTM1/p62 were clearly observable at higher exposures between MOI 1 and MOI 10 (Figure S1A, first panel, lanes 3 to 5), consistent with Figure 1A. Furthermore, we analyzed ICP27, ICP8, and ICP5 levels to validate the in vitro infection (Figure S1A, sixth to eighth panels). Here, SQSTM1 is linked to infection.