WT Sp infection of monolayers pre-treated with CDC failed to trigger PMN transmigration (Fig. 1a, “WT + CDC”), suggesting that PMN transmigration across Sp-infected ALI monolayers was dependent on eicosanoid lipid mediators produced by 12-LOX, recapitulating our findings during pulmonary Sp challenge in mice. The gene discussed is ALOX12; the disease is infection.