To delve into the underlying mechanisms of these heteroaromatic N‐ oxides' antitumor effects, we conducted western blot (WB) experiments to detect key proteins involved in apoptotic pathways (Caspase‐3, BCL‐2), autophagy pathways (LC3, P62), and tumor metastasis (N‐cadherin, MMP2) in MDA‐MB‐231 and MDA‐MB‐468 cells.[14] The results revealed that these compounds, particularly 3e, significantly enhanced cellular apoptosis and autophagy while inhibiting tumor metastasis (Figure 5b and Figure S1b, Supporting Information). This evidence concerns the gene MAP1LC3A and neoplasm.