Immunohistochemical analysis consistently demonstrated marked reduction in α‐SMA (a fibrosis marker) and type I collagen (COL‐1) expression levels within the kidney at day 28 post‐AKI following treatment with MCM@MOF@irisin compared with saline‐ or irisin‐treated renal I/R‐injured mice (Figure 6d,e). This evidence concerns the gene FNDC5 and acute kidney injury.