SEdb SE‐based TF‐GENE analysis program predicted TCF4 to be regulated by TFs that are critical for controlling early stages of SNS development including the master regulator of early SNS commitment paired‐like homeobox 2B gene (PHOX2B), a core MES TFs paired related homeobox 1 (PRRX1), pioneer TFs gata binding proteins (GATA2 and 3), and TFAP2 TFs, which are CRC independent but are important early SNS factors (Fig. 1B and Fig. S1c). This evidence concerns the gene PRRX1 and colorectal carcinoma.