Further analysis indicated that high ALYREF expression was an independent adverse prognostic factor for DMFS (HR, 3.19; 95% CI, 1.14–8.92; P = 0.027) and OS (HR, 2.79; 95% CI, 1.07–5.42; P = 0.047) in NPC patients. This evidence concerns the gene ALYREF and nasopharyngeal carcinoma.