In line with the result that the FA pathway is impaired in human KMT2D-deficient HNSCC upon 2-DG treatment, IHC staining for FANCD2 in 4NQO-induced mouse HNSCC also revealed that the protein levels of FANCD2 were decreased after 2-DG treatment in Kmt2d-HT HNSCC (Supplementary Fig. 5c). Here, FANCD2 is linked to head and neck squamous cell carcinoma.