Based on the result that the FA pathway is impaired in KMT2D-KO HNSCC cells after treatment of 2-DG, we next examined whether the combination of 2-DG and low-dose of DNA crosslinking agents would collaboratively induce apoptosis in KMT2D-deficient HNSCC cells. The gene discussed is KMT2D; the disease is head and neck squamous cell carcinoma.