To investigate the role of KMT2D in HNSCC tumorigenesis, we established Kmt2d heterozygous conditional knockout mouse (K14creER;Kmt2dfl/+: Kmt2d-HT) and then treated these mice together with wildtype mice (K14creER;Kmt2d+/+: Kmt2d-WT) with 4-nitroquinoline 1-oxide (4NQO) to induce SCC in the tongue. The gene discussed is KMT2D; the disease is head and neck squamous cell carcinoma.