We adoptively transferred pmel-1 T cells overexpressing Lmo4-Thy1.1 or Thy1.1 control into wild-type mice bearing B16 melanomas carrying the mutated gp100 epitope (KVP).30 To enhance the expansion and function of transferred T cells, we co-delivered a gp100-vv vaccine intravenously and administered high doses of IL-2 intraperitoneally (Fig. 3a). This evidence concerns the gene PMEL and melanoma.