To further determine if STAT3 signaling is also required for the antitumor effects of LMO4-overexpressing T cells, we adoptively transferred pmel-1Cas9 Lmo4-Thy1.1 and Thy1.1 T cells nucleofected with gRNAs specific for Stat3 or Thy1.2 into mice bearing subcutaneous B16KVP melanomas in conjunction with gp100-vv and IL-2. Here, PMEL is linked to melanoma.