Additionally, we generated a GSPT1 knockout (GSPT1-KO) U87 glioblastoma cell line by genome editing along with a rescued GSPT1-KO U87 glioblastoma cell line with stable overexpression of FLAG-tagged GSPT1 (Rescued GSPT1-KO) and examined the effects of GSPT1 using these cells and the transplantation brain tumor model. This evidence concerns the gene GSPT1 and glioblastoma.