PTBP3, also known as “regulator of differentiation 1”, is a member of the polypyrimidine bundle binding protein family.[13] PTBP3 plays important roles in RNA splicing, translational activation, and mRNA stabilization.[14] Recent studies have shown that PTBP3 acts as an oncogene and promotes the progression of hepatocellular carcinoma, gastric cancer, and colorectal cancer.[15, 16, 17] Here, using bioinformatics screening, we discovered that PTBP3 was highly expressed in gallbladder cancer, but had no significant effect on the biological behavior of tumor cells. The gene discussed is PTBP3; the disease is hepatocellular carcinoma.