Consistent with our findings in patients with gallbladder cancer, PTBP3 is highly expressed in multiple tumors and is associated with poor prognosis.[14, 16, 47, 48] PTBP3 can maintain UBE4A mRNA stability to promote colorectal cancer cell proliferation[14] and can also promote gastric cancer metastasis by mediating alternative splicing in CAV1.[15] Interestingly, we found that knockdown of PTBP3 did not significantly alter the biological behavior of gallbladder cancer cells. The gene discussed is UBE4A; the disease is gallbladder cancer.