Dysregulation of immune checkpoints plays an important role in immune evasion in many malignant tumors.[30] Among them, disruption of the PD‐1 /PD‐L1 axis was shown to be significantly associated with tumor progression.[31] The results of the mRNA‐seq analysis demonstrated that the expression of PTBP3 did not significantly correlate with PD‐L1 expression, but did positively correlate with PD‐1 expression in the 12 paired gallbladder cancer samples (Figure S3D, Supporting Information). Here, PTBP3 is linked to gallbladder cancer.