PTBP3, also known as “regulator of differentiation 1”, is a member of the polypyrimidine bundle binding protein family.[13] PTBP3 plays important roles in RNA splicing, translational activation, and mRNA stabilization.[14] Recent studies have shown that PTBP3 acts as an oncogene and promotes the progression of hepatocellular carcinoma, gastric cancer, and colorectal cancer.[15, 16, 17] Here, using bioinformatics screening, we discovered that PTBP3 was highly expressed in gallbladder cancer, but had no significant effect on the biological behavior of tumor cells. This evidence concerns the gene PTBP3 and neoplasm.